Which oil used for DLC test?

Examine the stained smear under the low power objective (10X) in the microscope for screening purpose and Chooses the proper portion of the smear. Placed one drop of Cedar wood oil (immersion oil) on the smear.

Which fluid is used in DLC?

Differential leucocyte count measures the percentage of each type of white blood cell present in the given sample. A differential count also can detect immature WBCs and other abnormalities. The fluid samples are taken from cavities in the body like urine, CSF, etc.

Which stain is used in DLC test?

Procedure of manual method:

1- Preparing the blood film by using Giemza or Leishman stain. 2- Choosing the field of test so that it is monolayer. 3- Microscopic examination (40X then by oil lens) and record the number of all WBC types that observed.

What stain is used for differential leukocyte count?

To determine the differential, a drop of blood is thinly spread over a glass slide, air dried, and stained with a Romanofsky stain, most commonly the Wright or May-Grunewald-Giemsa technique.

What is the limit for DLC test?

The DLC blood test normal range are: Neutrophils – 2500-8000 per mm3 (55-70%) Lymphocytes – 1000-4000 per mm3 (20–40%) Monocytes – 100-700 per mm3 (2–8%)

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How to prepare smear in DLC?

Which stain is used for TLC and DLC?

Thin-layer chromatography (TLC) was done by manual method (hemocytometer method) using improved Neubauer Chamber. For calculating DLC smear was made from the EDTA blood and was stained by Leishman stain (Romanowsky group).

Which stain is used in differential staining?

What is Differential Staining? Differential staining is a procedure where more than one dye is used to differentiate between different types of microorganisms on a slide. This type of staining helps to differentiate between cell types and cell structures.

What is used in differential stain?

First cells are stained with crystal violet, followed by the addition of a setting agent for the stain (iodine). Then alcohol is applied, which selectively removes the stain from only the Gram negative cells. Finally, a secondary stain, safranin, is added, which counterstains the decolorized cells pink.

What dyes are used in DLC?

The polychromic staining solution (Leishman stain) contains methylene blue and eosin. These basic and acidic dyes induce multiple colors when applied to cells. Methanol acts a fixative and also as a solvent. The fixative does not make them adhere to the glass slide.

Why Leishman stain is used in DLC?

The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step. Leishman stain is commonly used when there is need to examine the Blood smear for the Various blood cells, Differential Leucocyte count, Type of Anemia, Toxic Granules & Platelet count etc.

What are the different stains used for DLC?

What is TLC DLC test for fluid?

TLC & DLC test is a regular complete blood test that measures various components of the blood. It provides information about a person's general health. Hb measures the number of red blood cells and TLC measures the total number of white blood cells, with a high white blood cell count indicating infection.

What is the ratio of cement in DLC?

Concrete Subbase Course(DLC) mixing ratio:

As per MoRTH, the mix shall be proportioned with a maximum aggregate cement ratio of 15:1.

What are the 3 types of differential staining?

Differential stains use more than one stain, and cells will have a different appearance based on their chemical or structural properties. Some examples of differential stains are the Gram stain, acid-fast stain, and endospore stain.

What reagent is used in differential staining?

Differential staining uses more than one staining reagent (dye) to differentiate cellular structures. Streptococcus, Escherichia coli culture, methylene blue reagent, glass slide, inoculation loop, Bunsen burner, blotting paper, pasture pipette, staining tray, binocular microscope.

What are the 4 differential stains?

Examples of differential staining include gram staining, endospore staining, acid-fast staining, and metachromatic staining.

What is the best solvent for TLC?

Which type of solvents are used in TLC?

Solvent (Mobile Phase) Proper solvent selection is perhaps the most important aspect of TLC, and determining the best solvent may require a degree of trial and error. As with plate selection, keep in mind the chemical properties of the analytes. A common starting solvent is 1:1 hexane:ethyl acetate.

What is difference between TLC and DLC?

Hb measures the number of red blood cells, TLC measures the total number of white blood cells, which if high in number indicates an infection, DLC measures the consistency and structure of the white blood cells and counts the percentage of basophils, eosinophils, neutrophils, monocytes and lymphocytes.

What is the principle of DLC?

PRINCIPLE: A differential white blood cell count is performed to determine the percentage of each of the various types of white blood cells present in a blood sample. The test is useful because the relative proportions of white blood cells may change in particular diseases.

What are the 3 methods of smear preparation?

Four different types of smear preparation methods (conventional method, blood film method, drop and rest method, and water-wash method) were carried out according to the standard reference as described below.